Table II.

ΔCT values ± Δ standard deviation (normalized to GAPDH) and corresponding percent expression (mean ± standard deviation) of transcripts of interest in inflammatory media conditions relative to control media

Transcript	Control media		LPS media		rhIL-1β media
	ΔCT ± ΔSD	% ± SD	ΔCT ± ΔSD	% ± SD	ΔCT ± ΔSD	% ± SD
IL-1β	2.82 ± 0.12	100 ± 8.67	1.22 ± 0.10	303.41 ± 20.26 (P< 0.001)	3.60 ± 0.14	58.24 ± 5.39 (P =0.007)
TNF-α	7.22 ± 0.14	100 ± 9.38	1.20 ± 0.10	6,489.34 ± 434.57 (P< 0.001)	2.22 ± 0.12	3,244.67 ± 263.10 (P< 0.001)
IL-2	ND	–	ND	–	8.66 ± 0.14	NA
IL-8	4.62 ± 0.13	100 ± 8.72	0.11 ± 0.10	2,294.33 ± 168.74 (P< 0.001)	1.11 ± 0.11	1,147.16 ± 84.21 (P< 0.001)
IL-12	5.10 ± 0.14	100 ± 9.63	ND	–	4.70 ± 0.14	131.19 ± 11.45 (P =0.009)
TGF-β	−4.91 ± 0.10	100 ± 7.17	−3.50 ± 0.12	37.89 ± 3.03 (P =0.003)	−3.12 ± 0.10	28.71 ± 1.92 (P =0.002)
MMP13	2.94 ± 0.11	100 ± 7.92	−3.19 ± 0.08	7,003.48 ± 377.79 (P< 0.001)	−1.4 ± 0.10	2,082.15 ± 139.44 (P< 0.001)
MMP2	ND	–	4.32 ± 0.11	NA	6.00 ± 0.10	NA
IFN-γ	−8.12 ± 0.14	100 ± 9.25	−8.71 ± 0.02	151.57 ± 2.09 (P =0.005)	−9.60 ± 0.08	282.84 ± 15.26 (P< 0.001)
iNOS	3.16 ± 0.14	100 ± 9.34	2.42 ± 0.08	167.01 ± 9.01 (P =0.003)	2.22 ± 0.11	191.85 ± 14.09 (P< 0.001)

ND =Amplicon not detected; NA= Comparative CT(2^−ΔΔCT) method not applicable; P values are indicated.