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. Author manuscript; available in PMC: 2012 Sep 23.
Published in final edited form as: J Chromatogr A. 2011 Jul 24;1218(38):6675–6687. doi: 10.1016/j.chroma.2011.07.063

Table 1.

Elution conditions.

1DLC or the first dimension in LC × LC Second dimension in LC × LC
Elution Condition 1
A: 1.5 mM H3PO4 in water; B: ACN A: 10 mM H3PO4 in water; B: ACN
Time (min) B% Time (min) %B
 0 0 0 0
 24 50 0.30 100
 24.01 0 0.31 0
Elution Condition 2
A: 10 mM HClO4 in water; B: ACN A: 10 mM H3PO4 in water; B: ACN
Time (min) B% Time (min) %B
 0 0 0 0
 24 50 0.30 100
 24.01 0 0.31 0