. Author manuscript; available in PMC: 2012 Sep 23.

Published in final edited form as: J Chromatogr A. 2011 Jul 24;1218(38):6675–6687. doi: 10.1016/j.chroma.2011.07.063

Table 4.

LC × LC results using various samples.

Column

n_{c, 2 D}^{*}

f_coverage^d

n_{c, 2 D}^{'}

Peaks_1D^f

Peaks_2D^g

r²^h

Whole grain bread extract samples under Elution Condition 1

C3^a

1172

0.40

469

0.223

Bonus^b

1147

0.33

379

0.320

Juice extract samples under Elution Condition 1

C3^a

1172

0.25

293

0.376

Bonus^b

1147

0.31

356

0.173

Yeast supernatant samples under Elution Condition 1

C3^a

1172

0.40

469

128

0.494

Bonus^b

1147

0.40

459

111

0.477

Human urine extract samples under Elution Condition 2

C3^a

1172

0.36

422

104

0.396

Bonus^b

1147

0.33

379

0.451

C3: Zorbax 300SB-C3 (3.5 μ particles).

Bonus: Zorbax Bonus-RP (3.5 μ particles).

$n_{c, 2 D}^{*}$ : Corrected LC × LC peak capacity.

f_coverage: Coverage factor.

$n_{c, 2 D}^{'}$ : Effective LC × LC peak capacity.

Peaks_1D: Peak number counted from 1DLC chromatograms.

Peaks_2D: Peak number counted from LC × LC chromatograms.

r²: Coefficient of determination of peaks on LC × LC chromatograms.