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. 2011 Nov 21;208(12):2511–2524. doi: 10.1084/jem.20102545

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Figure 2. — LN SCS and medullary macrophages are infected by VV. (A) Flow cytometric analysis of single-cell LN suspensions from saline-injected mice. CD169⁺CD11c^lo macrophages can be further divided into CD11b⁺F4/80⁻ SCS or CD11b⁺F4/80⁺ medullary macrophages. (B) LNs from VV-infected mice 10 hpi. Cells were gated based on expression of a vaccinia-expressed fluorescent protein, into DCs and CD169⁺ macrophages, and finally on F4/80⁺ or F4/80⁻ cells. (C) Numbers of vaccinia-infected cells in different nodal populations. Circles indicate individual LNs, and bars show mean. (D) Whole-mount confocal images of LNs 10 hpi, with VV-NP-S-mCherry (red). Macrophages, green (FITC-labeled dextran); F4/80, grey. Scale bars are shown in micrometers. Data are representative of three experiments.