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. 2011 Nov 21;208(12):2417–2427. doi: 10.1084/jem.20111688

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© 2011 Ivanovs et al.

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Figure 3. — Human AGM region-derived HSCs provide long-term multilineage hematopoietic engraftment upon retransplantation into secondary recipients. Representative flow cytometry plots show human B (CD19⁺), T (CD3⁺), and myeloid cells (CD33⁺) in the peripheral blood (A) and BM (B) of an NSG mouse transplanted with BM from a primary recipient engrafted with HSCs from a CS 15 AGM region. Secondary transplantation was performed 7 mo after primary transplantation. The analysis of secondary recipients was performed 7 mo later. Eight independent secondary transplantation experiments were performed.