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. Author manuscript; available in PMC: 2012 Jan 12.
Published in final edited form as: Adv Exp Med Biol. 2011;720:121–134. doi: 10.1007/978-1-4614-0254-1_10

Figure 4.

Figure 4

Expression profile of EMT markers and their regulators during malignant cell transformation.

A. A list of EMT markers and promoting genes was generated a priory by literature search (26). Hierarchical clustering of cell lines and genes was performed using dChip software. Two sample clusters (κ and λ) and two gene clusters (α and β) were identified. The red, white, and blue colors represent level above, at, and below mean expression, respectively. B. Detection of epithelial and mesenchymal markers by immunochytochemistry (100x). a: Histological sections of MCF-10F cells, reacted with pre-immune mouse serum, were used as the negative control; b, c, d: MCF-10F reacted for EMA, E-Cadherin, vimentin, respectively; e: trMCF cells reacted with pre-immune mouse serum used as negative control; f, g, h: trMCF cells reacted for EMA, E-cadherin and vimentin, respectively; i: bsMCF cells reacted with pre-immune mouse serum as a negative control; j, k, l: bsMCF cells reacted for EMA, E-cadherin and vimentin, respectively; m: caMCF tumor cell line cells reacted with pre-immune mouse serum used as negative control; n, o, p: caMCF tumor cell lines reacted for EMA, E-cadherin and vimentin, respectively; q and r: invasive ductal carcinoma of the breast as positive control and immunoreacted for EMA and E-cadherin, respectively; s: histological section of an invasive adenocarcinoma immunoreacted for vimentin. From: Huang, Y., Fernandez, S., Goodwin, S., Russo, P.A., Russo, I. H., Sutter, T., and Russo, J. Epithelial to Mesenchymal Transition in Human Breast Epithelial Cells Transformed by 17- beta- Estradiol. Cancer Res 67 11147–11157, 2007.