Table 1.
Protein | Activation wavelength (nm)a |
Stateb | Excitation wavelength (nm) |
Emission wavelength (nm) |
Contrast (fold increase) | Reference |
---|---|---|---|---|---|---|
PA-GFP | 405 | Pre | 400 | 515 | 70 | Patterson and Lippincott-Schwartz (2002) |
Post | 504 | 517 | ||||
PS-CFP | 405 | Pre | 402 | 468 | 300 green 1500 green-to-cyan ratio | Chudakov et al. (2004) |
Post | 490 | 511 | ||||
PS-CFP2 | 405 | Pre | 400 | 470 | See supplier's Web site | |
Post | 490 | 511 | ||||
PAmRFP1-1 | 405 | Post | 578 | 605 | 70 | Verkhusha and Sorkin (2005) |
PAmCherry1 | 405 | Post | 564 | 595 | Subach et al. (2009) | |
PATagRFP | 405 | Post | 562 | 595 | Subach et al. (2010) | |
Dronpac | 490, 405 | Pre | 503 | 518 | Ando et al. (2004) | |
Dronpa-2 | 490, 405 | ON | 486 | 513 | Ando et al. (2007) | |
Dronpa-3 | 490, 405 | ON | 487 | 514 | Ando et al. (2007) | |
rsFastlime | 490, 405 | ON | 496 | 518 | 67 | Stiel et al. (2007) |
Padron | 490, 405 | ON | 503 | 522 | 143 | Andresen et al. (2008) |
bsDronpa | 490, 405 | ON | 460 | 504 | 17 | Andresen et al. (2008) |
rsCherry | 470, 561 | ON | 572 | 610 | 6.7 | Stiel et al. (2008) |
rsCherryRev | 470, 561 | ON | 572 | 608 | 20 | Stiel et al. (2008) |
KFP1 | 532 | Post | 580 | 600 | Chudakov et al. (2003) | |
Kaede | 405 | Pre | 508 | 518 | 2000 red-to-green ratio | Ando et al. (2002) |
Post | 572 | 582 | ||||
Kikume Green-Red (KikGR) | 405 | Pre | 507 | 517 | 2000 red-to-green ratio | Tsutsui et al. (2005) |
Post | 583 | 593 | ||||
mKikGR | 405 | Pre | 505 | 515 | Habuchi et al. (2008) | |
Post | 580 | 591 | ||||
EosFP | 405 | Pre | 506 | 516 | Wiedenmann et al. (2004) | |
Post | 571 | 581 | ||||
mEosFP | 405 | Pre | 505 | 516 | Wiedenmann et al. (2004) | |
Post | 573 | 584 | ||||
IrisFP | 405 photoconversion | Pre | 488 | 516 | Adam et al. (2008) | |
488 or 561 photoswitching | Post | 551 | 580 | |||
Dendra | 405 or 488 | Pre | 486 | 505 | 4500 red-to-green ratio | Gurskaya et al. (2006) |
Post | 558 | 575 | ||||
Dendra2 | 405 or 488 | Pre | 486 | 505 | See supplier's Web site | |
Post | 558 | 575 |
The wavelength range for activation can be broad for each protein. The wavelengths listed here are suggestions based on commonly available laser lines.
The wavelengths to the right of Pre represent the major peaks prior to photoactivation. The wavelengths to the right of Post represent the major peaks after photoactivation; absence of Pre wavelengths indicates that the protein is not initially fluorescent (values not published).
Dronpa displays green fluorescence with excitation at 503 nm and emission at 518 nm. Excitation at 490 nm results in loss of absorbance at 503 nm. Upon irradiation at 400 nm, green fluorescence is restored.