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. Author manuscript; available in PMC: 2012 Jul 1.
Published in final edited form as: Curr Protoc Cytom. 2011 Jul;CHAPTER:Unit–12.23. doi: 10.1002/0471142956.cy1223s57

Table 1.

Protein Activation wavelength
(nm)a
Stateb Excitation wavelength
(nm)
Emission wavelength
(nm)
Contrast (fold increase) Reference
PA-GFP 405 Pre 400 515 70 Patterson and Lippincott-Schwartz (2002)
Post 504 517
PS-CFP 405 Pre 402 468 300 green 1500 green-to-cyan ratio Chudakov et al. (2004)
Post 490 511
PS-CFP2 405 Pre 400 470 See supplier's Web site
Post 490 511
PAmRFP1-1 405 Post 578 605 70 Verkhusha and Sorkin (2005)
PAmCherry1 405 Post 564 595 Subach et al. (2009)
PATagRFP 405 Post 562 595 Subach et al. (2010)
Dronpac 490, 405 Pre 503 518 Ando et al. (2004)
Dronpa-2 490, 405 ON 486 513 Ando et al. (2007)
Dronpa-3 490, 405 ON 487 514 Ando et al. (2007)
rsFastlime 490, 405 ON 496 518 67 Stiel et al. (2007)
Padron 490, 405 ON 503 522 143 Andresen et al. (2008)
bsDronpa 490, 405 ON 460 504 17 Andresen et al. (2008)
rsCherry 470, 561 ON 572 610 6.7 Stiel et al. (2008)
rsCherryRev 470, 561 ON 572 608 20 Stiel et al. (2008)
KFP1 532 Post 580 600 Chudakov et al. (2003)
Kaede 405 Pre 508 518 2000 red-to-green ratio Ando et al. (2002)
Post 572 582
Kikume Green-Red (KikGR) 405 Pre 507 517 2000 red-to-green ratio Tsutsui et al. (2005)
Post 583 593
mKikGR 405 Pre 505 515 Habuchi et al. (2008)
Post 580 591
EosFP 405 Pre 506 516 Wiedenmann et al. (2004)
Post 571 581
mEosFP 405 Pre 505 516 Wiedenmann et al. (2004)
Post 573 584
IrisFP 405 photoconversion Pre 488 516 Adam et al. (2008)
488 or 561 photoswitching Post 551 580
Dendra 405 or 488 Pre 486 505 4500 red-to-green ratio Gurskaya et al. (2006)
Post 558 575
Dendra2 405 or 488 Pre 486 505 See supplier's Web site
Post 558 575
a

The wavelength range for activation can be broad for each protein. The wavelengths listed here are suggestions based on commonly available laser lines.

b

The wavelengths to the right of Pre represent the major peaks prior to photoactivation. The wavelengths to the right of Post represent the major peaks after photoactivation; absence of Pre wavelengths indicates that the protein is not initially fluorescent (values not published).

c

Dronpa displays green fluorescence with excitation at 503 nm and emission at 518 nm. Excitation at 490 nm results in loss of absorbance at 503 nm. Upon irradiation at 400 nm, green fluorescence is restored.