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. 2011 Nov 29;12(12):8539–8561. doi: 10.3390/ijms12128539

Figure 5.

Figure 5

Zfh1 is required for a subset of motor axons to emerge from the spinal cord. (A,B) Flat-mounted zebrafish embryos were labeled with either anti-FASIII (A,B) or anti-myc (C,D) to visualize the axonal projections of an early-developing intersegmental nerve (ISN) called ISNb. Note, ISNb axons selectively express the isl-tau:myc transgene and montages were created by combining images taken from multiple focal planes to visualize the extent of the ISNb nerve. In stage 16 (A) and 15 (C) wild type embryos, zfh1-expressing RP MNs (white asterisk) form an ISNb nerve that extends out and beyond the lateral edge of the CNS (dashed white line). White arrowheads correspond to the terminal end of an ISNb axon. In contrast, a subset of RP MN axons fails to exit the CNS in zfh1 mutants (B,D, top), while others extend appropriately to their peripheral muscle field (B,D, bottom) [20].