Figure 5.

Effects of cinnarizine (A) and trazodone (B) on murine tyrosinase activity. Tyrosinase activity was determined by spectrometry, using l-DOPA as a substrate, as described in the experimental section. B16 cells were cultivated for 5 d and lysed to obtain a crude tyrosinase extract. The cell-free tyrosinase activity was determined by directly mixing the crude tyrosinase, l-DOPA, and various concentrations of cinnarizine and trazodone. Averaged data (n = 3) are presented with error bars indicating SD. A value of p < 0.001 (*), obtained with a Student’s t-test by comparing the data with that of control, was considered statistically significant.