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. 2011 Dec 8;12(12):9108–9124. doi: 10.3390/ijms12129108

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© 2011 by the authors; licensee MDPI, Basel, Switzerland.

This article is an open-access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).

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(a) Amplification times for LAMP without Loop primers (blue) and STEM-LAMP with Stem primers instead of Loop primers (pink) for 10, 1000 and 100,000 copies of the HIV-AT construct (restricted with Kpn1 and pre-denatured); STEM-LAMP without displacement primers for the same copy number is shown in green; (b) A six log dilution series of the HIV-AT construct using STEM-LAMP (all dilutions were measured and detected in triplicate unless indicated otherwise). Final primers concentrations were: sHIV-BIP, sHIV-FIP, HIV-StemB and HIV-StemF—1.6 μM each, HIV-DisplB and HIV-DisplF—0.4 μM each. Each set of data included three NTCs that remained clean throughout the duration of the assay and were not associated with any time-to-peak value (hence not shown graphically). Error bars represent standard deviations of triplicate measurements.