Figure 5.

Schematic diagram of all priming sites used in Stem-accelerated LAMP using Loop primers (LAMP-LOOP) (a); comparison of LAMP-LOOP with inlA-AT target in the absence (lime) and presence (pink) of Stem primers (b) over a six log dilution series (all dilutions were measured and detected in triplicate unless indicated otherwise). Final primers concentrations were: Lm-BIP, Lm-FIP, Lm-StemB, Lm-StemF, Lm-LoopB, Lm-LoopF, Lm-DisplB and Lm-DispIF—0.8 μM each. Each set of data included three NTCs that remained clean throughout the duration of the assay and were not associated with any time-to-peak value (hence not shown graphically). Error bars represent standard deviations of triplicate measurements.