Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2011 Dec 8;12(12):9108–9124. doi: 10.3390/ijms12129108

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2011 by the authors; licensee MDPI, Basel, Switzerland.

This article is an open-access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).

PMC Copyright notice

Schematic diagram of the “forward” (conventional) and “reverse” orientation of Loop primers in LAMP-LOOP (a); Stem primers in STEM-LAMP (b) and comparison of LAMP-LOOP with Loop primers in conventional (lime) and reverse (orange) orientation (c) and STEM-LAMP with Stem primers in conventional (pink) and reverse (purple) orientation (d) for 10, 1000 and 100,000 copies of inlA-AT construct (all dilutions were measured and detected in triplicate unless indicated otherwise). Final primers concentrations were: Lm-BIP, Lm-FIP, Lm-StemB, Lm-StemF, Lm-LoopB, Lm-LoopF, rLm-StemB, rLm-StemF, rLm-LoopB, rLm-LoopF, Lm-DisplB and Lm-DisplF—0.8 μM each. Each set of data included three NTCs that remained clean throughout the duration of the assay and were not associated with any time-to-peak value (hence not shown graphically). Error bars represent standard deviations of triplicate measurements.

Schematic diagram of the “forward” (conventional) and “reverse” orientation of Loop primers in LAMP-LOOP (a); Stem primers in STEM-LAMP (b) and comparison of LAMP-LOOP with Loop primers in conventional (lime) and reverse (orange) orientation (c) and STEM-LAMP with Stem primers in conventional (pink) and reverse (purple) orientation (d) for 10, 1000 and 100,000 copies of inlA-AT construct (all dilutions were measured and detected in triplicate unless indicated otherwise). Final primers concentrations were: Lm-BIP, Lm-FIP, Lm-StemB, Lm-StemF, Lm-LoopB, Lm-LoopF, rLm-StemB, rLm-StemF, rLm-LoopB, rLm-LoopF, Lm-DisplB and Lm-DisplF—0.8 μM each. Each set of data included three NTCs that remained clean throughout the duration of the assay and were not associated with any time-to-peak value (hence not shown graphically). Error bars represent standard deviations of triplicate measurements.