Figure 8.
Examples of the reverse primer orientation feasibility: (a) avoidance of mismatches in close proximity to 3′-end; (b) avoidance of hairpin formation at 3′-end; (c) avoidance of self- or hetero-primer-dimerization at 3′-end.
Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.