Figure 4.
Effects of inhibitors of swelling-activated anion channels and K-Cl cotransporter on volume regulation of mouse thymocytes. (A) Time courses of cell volume change in isotonic conditions (filled circles) and in hypotonic conditions in the absence (open circles) and presence of 50 μM Gd3+ (open triangles), 50 μM phloretin (open squares) and 250 μM glibenclamide (open diamonds). (B) Time courses of cell volume change in isotonic conditions (filled circles) and in hypotonic conditions in the absence (open circles) and presence of 1 μM and 10 μM DIOA (open triangles and open squares, respectively). The cell volume was measured by Coulter-type cell sizing method.