Figure 1.

Detection of insulin monomer, oligomer, and higher molecular weight aggregation states using UV-CE. Insulin was aggregated under agitation (185 rpm) at 0.2 mg/mL in 40 mM Tris (pH 8.0) containing 150 mM NaCl and at 25 °C. At 0 h (panel A), 4 h (panel B), 8 h (panel C), 12 h (panel D), and 24 h (panel E), CE was performed in conjunction with UV detection with a 0.5 psi pressure injection for 8 s with separation at 15 kV using 0.5% PHEA separation matrix in a PHEA coated capillary. Results are representative of three independent experiments. Supplementary data confirms the absence of significant peaks at a migration time of >180 min for 0, 4, and 8 h time points.