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. 2011 Dec 14;12(12):9369–9388. doi: 10.3390/ijms12129369

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© 2011 by the authors; licensee MDPI, Basel, Switzerland.

This article is an open-access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).

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Coaggregation of FITC-labeled insulin and unlabeled insulin. Insulin solutions consisting of 25% FITC-labeled insulin and 75% unlabeled insulin with LIF detection ( , n = 6) and UV detection ( , n = 3) were prepared at a concentration of 0.2 mg/mL in 40 mM Tris (pH 8.0) containing 150 mM NaCl. Solutions were subjected to agitation (185 rpm), and the formation of aggregates was monitored. LIF-CE was performed with a sample injection at 12 kV for 12 s with 15 kV separation and UV-CE was performed with a sample injection at 10 kV for 12 s with 15 kV separation. Both separations were performed using 1% PHEA separation matrix in PHEA coated capillary. Migration times were normalized as described in Figure 2. Error bars represent SE, n = 3–6. Some error bars lie within symbols. For all time points >0 h, UV data was statistically different from the LIF data with a p < 0.015.

Inline graphic — Coaggregation of FITC-labeled insulin and unlabeled insulin. Insulin solutions consisting of 25% FITC-labeled insulin and 75% unlabeled insulin with LIF detection ( , n = 6) and UV detection ( , n = 3) were prepared at a concentration of 0.2 mg/mL in 40 mM Tris (pH 8.0) containing 150 mM NaCl. Solutions were subjected to agitation (185 rpm), and the formation of aggregates was monitored. LIF-CE was performed with a sample injection at 12 kV for 12 s with 15 kV separation and UV-CE was performed with a sample injection at 10 kV for 12 s with 15 kV separation. Both separations were performed using 1% PHEA separation matrix in PHEA coated capillary. Migration times were normalized as described in Figure 2. Error bars represent SE, n = 3–6. Some error bars lie within symbols. For all time points >0 h, UV data was statistically different from the LIF data with a p < 0.015.