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. 2011 Dec 19;12(12):9471–9480. doi: 10.3390/ijms12129471

Table 1.

Primers used for PCR and sequencing of the SRD5A2 gene.

Exon Forward Primer Reverse Primer Ta* (°C) Fragment size (pb)
1 GCAGCGGCCCACCGGCGAGGAACA TGGACGCCGGGAGCAGGGCAGT 66 369
2 CAGTGAATCCTAACCTTTCCTCCC TTGTTAGCTGGGAAGTAGGTGGAG 59.5 243
3 AAGCACCACAATCTGGACACAT CTCCAGGGAAGAGTGAGAGTCTG 59.5 203
4 CAATGATTGACCTTCCGATTCTTC GTTTGGAGAAGAAGAAAGCTACGT 63 238
5 TCAGCCACTGCTCCATTATATTTA TTGACAGTTTTCATCCAGCATTGTG 59.5 171
*

Ta = annealing temperatures used in PCRs.