Table 1.
Exon | Forward Primer | Reverse Primer | Ta* (°C) | Fragment size (pb) |
---|---|---|---|---|
1 | GCAGCGGCCCACCGGCGAGGAACA | TGGACGCCGGGAGCAGGGCAGT | 66 | 369 |
2 | CAGTGAATCCTAACCTTTCCTCCC | TTGTTAGCTGGGAAGTAGGTGGAG | 59.5 | 243 |
3 | AAGCACCACAATCTGGACACAT | CTCCAGGGAAGAGTGAGAGTCTG | 59.5 | 203 |
4 | CAATGATTGACCTTCCGATTCTTC | GTTTGGAGAAGAAGAAAGCTACGT | 63 | 238 |
5 | TCAGCCACTGCTCCATTATATTTA | TTGACAGTTTTCATCCAGCATTGTG | 59.5 | 171 |
Ta = annealing temperatures used in PCRs.