TABLE 1.
Thermal stabilities of the duplexes between S-and R-6′-Me-FHNA modified DNA and RNA.
| Oligonucleotidea | FHNA-T* | Tmb[°C] | ΔTm/mod[°C] |
|---|---|---|---|
| GCGTTTTTTGCT | DNA | 45.6 | — |
| GCGTT-T*-TTTGCT | S-6′-Me 9 | 46.5 | + 0.9 |
| GCGTT-T*T*-TTTGCT | S-6′-Me 9 | 48.2 | + 1.3 |
| GCGTT-T*-TTTGCT | R-6′-Me 10 | 42.3 | − 3.3 |
| GCGTT-T*T*-TTTGCT | R-6′-Me 10 | 40.7 | − 2.4 |
a
T* indicates a modified nucleotide.
b
Tm values (error ± 0.5°C) were measured at 4 μM oligo concentration in 10 mM sodium phosphate buffer (pH 7.2) containing 100 mM NaCl and 0.1 mM EDTA. The RNA complement was 5′-r(AGCAAAAAACGC)-3′.