Figure 5.
AMD3100 blocks CXCL12-CXCR4 binding in vivo. (A) Mice were injected intraperitoneally with 2.5 x 105 each of HeyA8-CXCL12-CG and NG-CXCR4 cells. Baseline images for Gaussia luciferase complementation and firefly luciferase expressed constitutively by NG-CXCR4 cells were obtained 7 days after injecting ovarian cancer cells. Mice then were treated for 3 days with AMD3100 or vehicle control before repeating imaging studies. Representative images for mice treated with AMD3100 or vehicle control are displayed. Scale bars denote pseudocolor displays for bioluminescence for Gaussia and firefly luciferase images, respectively. (B) Quantified data for Gaussia luciferase (GL) activity (n = 7–8 per group). Change in bioluminescence from posttreatment to pretreatment values was calculated for each mouse, and mean values ± SEM were presented. *P < .05.