Table 2.
Binding constants based on a molar concentration scale of TrCel7A and TrCel7A core to linear xylan oligosaccharides
| Xylan oligosaccharide | TrCel7A core (K) | TrCel7A (K) |
|---|---|---|
| Xylotriose (xyl)3 | > 1.0 × 103 | - |
| Xylotetraose (xyl)4 | 2.97 × 103 | - |
| Xylohexaose (xyl)5 | 3.44 × 103 | 3.44 × 103 |
| Xyloheptaose (xyl)7 | 7.43 × 103 | 5.76 × 103 |
| Xylooctaose/xylononaose, (xyl)8/(xyl)9 (1:1) | 1.19 × 104 | 9.20 × 104 |
| Xylooctaose/xylononaose/xylodecaose, (xyl)8/(xyl)9/(xyl)10 (1:1:1) | 2.91 × 105 | - |
| Low XOS (5 g/L and 8.4 mM, average 4 DP)a | 7.18 × 102 | 9.13 × 102 |
| High XOS (5 g/L and 4.4 mM, average 8 DP)a | 4.57 × 103 | 3.47 × 103 |
DP = degree of polymerisation; TrCel7A = cellobiohydrolase I; XOS = xylan oligosaccharide; - = no data. aEstimated on the basis of high-performance anion-exchange chromatography with pulsed amperometric detection traces and binding experiments (n = 3).