Table 3.
Para-nitrophenyl release of TrCel7A and TrCel7A core from pNP-lac as a single substrate at 50°C and 30°Ca
| Enzyme (temperature substrate) |
Activity in 1 hour | Activity in 1 hour (1 g/L low XOS) | Activity in 1 hour (1 g/L high XOS) |
|---|---|---|---|
| TrCel7A core, 50°C | 127 ± 5 μM pNPb 436 ± 11 μM cbb |
21% ± 4 pNP 61% ± 6 cb |
10% ± 2 pNP 44% ± 2 cb |
| TrCel7A, 50°C | 99 ± 3 μM pNP 695 ± 32 μM cb |
15% ± 2 pNP 96% ± 4 cb |
9% ± 1 pNP 81% ± 5 cb |
| TrCel7A core, 30°C | 50 ± 2 μM pNP 177 ± 6 μM cb |
21% ± 2 pNP 61% ± 7 cb |
10% ± 4 pNP 55% ± 4 cb |
| TrCel7A, 30°C | 38 ± 1 μM pNP 204 ± 10 μM cb |
15% ± 3 pNP 96% ± 2 cb |
9% ± 3 pNP 81% ± 2 cb |
cb: cellobiose-solubilised; pNP = para-nitrophenyl release; pNP-lac = para-nitrophenyl-β-D-lacoside; TrCel7A = cellobiohydrolase I; XOS = xylan oligosaccharide. aThe cellobiose release from phosphoric acid swollen cellulose (PASC) in the presence of pNP-lac by TrCel7A and TrCel7A core at 50°C and 30°C was measured in separate experiments. The deviations are the standard deviations of five independent experiments. bThe cellobiose release from PASC was independent of pNP-lac (Table 4).