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. 2012 Jan 12;7(1):e28568. doi: 10.1371/journal.pone.0028568

Figure 4. EIF2A phosphorylation in CCT020312-treated cells.

Figure 4

A) Detection of EIF2A phosphorylation following CCT020312 treatment. HT29 human colon cancer and MCF-7 human breast cancer cells were treated with 10 µM CCT020312 (+) or vehicle () for the times indicated. Lysates were prepared and immunoblot-analysis performed using antibodies to detect EIF2A and Ser51-phosphorylated EIF2A (P-S51EIF2A) as indicated. B) Structure-activity relationships. Representatives from different chemical series were tested using HT29 cells for their respective ability to activate EIF2A phosphorylation. C) Analogue structures and potency of analogues for reducing P-S608-pRB and growth.