Table 3. Comparison of the protein bands detected by anti-ureaplasma IgG antibodies in maternal serum when probed against whole ureaplasma protein extract or MBA-negative ureaplasma protein extract.
PROTEIN BANDS RECOGNISED BY MATERNAL SERUM WHEN PROBED AGAINST: | |||
ANIMAL # | TREATMENT GROUP | Whole ureaplasma protein extract | MBA-negative ureaplasma protein extract |
2 | Avirulent | 45 kDa, 87 kDa | 45 kDa |
29 | Avirulent | 80 kDa | N/A |
91 | Avirulent | 45 kDa | 45 kDa |
102 | Avirulent | 70 kDa | N/A |
42 | Virulent | 50 kDa | 45 kDa |
66 | Virulent | 55 kDa, 62 kDa, 87 kDa | N/A |
83 | Virulent | 45 kDa, 50 kDa | 45 kDa |
85 | Virulent | 45 kDa, 50 kDa, 55 kDa, 60 kDa | N/A |
96 | Virulent | 50 kDa, 55 kDa, 60 kDa | 45 kDa |
Those proteins indicated in bold were detected in western blots using both whole ureaplasma and MBA negative ureaplasma protein extracts. kDa = kilodaltons.