Table 3. Plant species list used for determining sensitivity of multiplex (FLPS, LF, TAT, TZ) and singleplex assays (Plantago, Apiaceae, Poaceae).
Plant species | Detection limits of plant DNA | Assay type | |
Plant DNA only | plus wireworm DNA | ||
Fagopyrum esculentum | 200 | 200 | FLPS |
Lolium perenne | 100 | 200 | |
Phaseolus coccineus | 200 | 400 | |
Sinapis alba | 100 | 200 | |
Lupinus angustifolius | 100 | 200 | LF |
Fagopyrum esculentum | 400 | 800 | |
Taraxacum officinale | 100 | 100 | TAT |
Achillea millefolium | 200 | 400 | |
Trifolium pratense | 100 | 100 | |
Trifolium repens | 100 | 200 | |
Triticum aestivum | 100 | 200 | TZ |
Zea mays | 100 | 200 | |
Plantago lanceolata | 100 | 100 | Plantago spp. |
Plantago lanceolata | 100 | 100 | |
Anthriscus sylvestris | 800 | 800 | Apiaceae |
Carum carvi | 100 | 100 | |
Heracleum sphondylium | 100 | 200 | |
Pimpinella major | 1,600 | 1,600 | |
Avena sativa | 100 | 200 | Poaceae |
Bromus hordeaceus | 100 | 200 | |
Dactylis glomerata | 100 | 200 | |
Digitaria ischaemum | 100 | 200 | |
Lolium perenne | 100 | 100 | |
Setaria viridis | 100 | 100 | |
Trisetum flavescens | 100 | 100 |
Lowest detection rates achieved are given in number of template copies.