Table 1. Spectroscopic properties of all proteins under various experimental conditions.
| Rayleigh Scattering at 350 nm | Turbidity at 350 nm | ThT fluorescence intensity at 485 nm | |||||||||
| Protein | pI | pH below pI+protein | pH below pI+SDS+protein | pH above pI+SDS+protein | pH below pI+protein | pH below pI+SDS+protein | pH above pI+SDS+protein | pH below pI+protein | pH below pI+SDS+protein | pH below pI+SDS | pH above pI+SDS+protein |
| Human Serum Albumin | 5.67 | 36.01 | 6191 | 61.22 | 0.01 | 1.62 | 0.01 | 2.17 | 39.80 | 4.24 | 3.12 |
| Porcine Serum Albumin | 5.89 | 27.38 | 6160 | 61.00 | 0.01 | 1.52 | 0.01 | 2.00 | 158.50 | 4.56 | 4.54 |
| Bovine Serum Albumin | 5.65 | 28.88 | 2754 | 59.00 | 0.01 | 1.49 | 0.01 | 2.02 | 32.90 | 3.84 | 3.99 |
| Sheep Serum Albumin | 5.63 | 30.10 | 2750 | 60.00 | 0.01 | 1.39 | 0.02 | 2.07 | 218.40 | 4.35 | 4.08 |
| Rabbit Serum Albumin | 5.70 | 42.45 | 2860 | 72.00 | 0.01 | 1.18 | 0.01 | 2.43 | 171.20 | 3.60 | 3.68 |
| Ovalbumin | 4.50 | 68.47 | 8341 | 47.75 | 0.01 | 2.61 | 0.01 | 2.76 | 52.97 | 3.92 | 4.30 |
| M. javanicus lipase | 5.90 | 75.86 | 386 | 45.00 | 0.01 | 0.16 | 0.01 | 2.04 | 16.11 | 4.42 | 5.88 |
| Lysozyme | 11.20 | 189.10 | 561 | 53.36 | 0.01 | 0.14 | 0.01 | 4.20 | 83.97 | 4.85 | 6.89 |
| Invertase | 3.40 | 268.20 | 793 | 78.00 | 0.01 | 0.16 | 0.02 | 7.33 | 84.78 | 3.96 | 4.79 |
| Hemoglobin | 7.10 | 56.41 | 2986 | 69.00 | 0.02 | 2.98 | 0.01 | 2.65 | 12.30 | 4.42 | 3.46 |
| Glucose oxidase | 4.94 | 249.30 | 4996 | 60.60 | 0.01 | 1.48 | 0.01 | 27.09 | 165.10 | 4.19 | 3.10 |
| Gelatin | 4.70 | 44.82 | 6828 | 146.80 | 0.01 | 1.45 | 0.01 | 1.94 | 25.38 | 4.10 | 3.23 |
| Fetuin | 5.21 | 25.77 | 532 | 59.16 | 0.01 | 0.13 | 0.01 | 1.89 | 52.75 | 3.92 | 3.23 |
| Concanavalin A | 5.43 | 222.60 | 2871 | 48.20 | 0.01 | 0.65 | 0.03 | 3.05 | 64.68 | 4.55 | 2.13 |
| α- lactalbumin | 4.40 | 31.31 | 1766 | 98.00 | 0.01 | 0.69 | 0.01 | 1.82 | 52.38 | 3.93 | 4.12 |
| Cobra toxin | 7.69 | 32.64 | 1250 | 74.00 | 0.01 | 0.12 | 0.01 | 1.98 | 30.69 | 4.55 | 3.98 |
| C.rugosa lipase | 4.70 | 101.10 | 1384 | 80.00 | 0.01 | 0.34 | 0.01 | 3.17 | 22.97 | 4.08 | 2.99 |
| β-lacto globulin | 5.20 | 109.40 | 2056 | 50.62 | 0.01 | 0.12 | 0.01 | 2.42 | 8.71 | 4.53 | 3.98 |
| Bovine liver catalase | 5.40 | 311.30 | 7349 | 111.00 | 0.02 | 3.11 | 0.01 | 11.22 | 22.63 | 4.80 | 4.79 |
| α-amylase | 5.90 | 85.83 | 843 | 46.86 | 0.01 | 0.17 | 0.01 | 8.77 | 28.07 | 4.35 | 6.89 |
| Conalbumin | 6.69 | 59.08 | 7433 | 51.66 | 0.02 | 2.17 | 0.01 | 1.96 | 75.02 | 4.33 | 5.44 |
| Chymopapain | 10.40 | 67.00 | 2309 | 69.00 | 0.01 | 0.36 | 0.01 | 5.90 | 54.04 | 3.98 | 4.34 |
| Banana lectin | 6.26 | 152.90 | 1812 | 78.00 | 0.01 | 0.41 | 0.01 | 1.77 | 44.37 | 4.49 | 4.57 |
| Rat IgG | 7.80 | 107.50 | 6525 | 63.00 | 0.01 | 2.01 | 0.01 | 2.42 | 50.82 | 4.37 | 5.46 |
| Asialofetuin | 5.10 | 36.23 | 1720 | 90.00 | 0.01 | 0.39 | 0.01 | 2.61 | 86.31 | 4.31 | 5.79 |