Figure 5. Both type I and II germ cells are eliminated by apoptosis in the sox9b mutant medaka.

(A–H) Ventral views of 10 dph medaka gonads immunostained with anti-OLVAS (germ cell marker, purple) and anti-cleaved caspase 3 (blue). Merged images (A, C, E and G) and cleaved caspase 3 signals (B, D, F and H) are shown. Low levels of germ cell apoptosis only were evident in wild-type XY and XX medaka at 10 dph. However, type I and type II germ cells (type I, arrows; type II, a bracket) were eliminated by apoptosis (Table 2) in the homozygous (E and F) and heterozygous (G and H) mutants. Scale bar, 50 µm.