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. 2012 Jan 12;7(1):e30229. doi: 10.1371/journal.pone.0030229

Figure 4. aAPC/mOKT3 expanded TIL are Foxp3 negative and secrete predominantly Th1 cytokines.

Figure 4

(A) Expansion of TIL obtained from breast and ovarian cancer ascites and melanoma metastases is shown. Shading indicates the proportion of CD4+ (white) and CD8+ (black) T cells in expanded cultures. The percentage of CD8+ T cells in pre- and post-expansion cultures is shown. Note that in all samples tested, the percentage of CD8+ T cells increased even in those that initially contained a minimal percentage of CD8+ T cells. NT denotes not tested. (B) CD4+ CD25+ Foxp3+ Treg cells, present pre-expansion, were not detectable after one month of culture. CD4+ CD25+ cells were intracellularly stained with anti-Foxp3 mAb (open) and isotype control (shaded). (C) IFN-γ, IL-2, IL-4, and IL-10 secretion of expanded TIL was determined by ELISPOT assays. Cytokine secretion by TIL from the breast cancer ascites specimen prior to expansion is shown as a control. Pre-expansion samples from melanoma and ovarian cancer specimens were not studied because of low initial cell numbers.