Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2012 Jan 12;7(1):e30251. doi: 10.1371/journal.pone.0030251

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Ord et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

PMC Copyright notice

Anti-EBA-175_RII antibodies are able to detect native EBA-175 protein of 175 kDa from 3D7 parasite lysates (Lane 1, panel A). Anti-EBA-175_RII/rRH5 (sera from the combined vaccine) is able to detect both EBA-175 and RH5 native proteins from the same lysate (Lane 2, panel A; EBA-175 indicated by top arrow; RH5 indicated by bottom arrow). Anti-rRH5 antibodies are specific for the native 65 kDa RH5 protein from 3D7 parasite lysate (panel B; indicated by arrow). All products were visualized with ECL after immunoblotting. Immunofluorescent detection of EBA-175 and RH5 in mature and rupturing 3D7 schizonts. Parasites were labelled with anti-EBA-175_RII (panel C) or with anti-rRH5 (panel D) at dilutions of 1∶100 and 1∶500, then the slides were incubated with FITC-conjugated anti-mouse IgG (green) and mounted with 10 mg/mL DAPI (blue). EBA-175 and RH5 are each localised to the apical end of merozoites. Non-staining with preimmune sera (1∶100 dilution) confirm specificity of each antibody to its respective antigen.