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. 2012 Jan 12;7(1):e30143. doi: 10.1371/journal.pone.0030143

Figure 3. NaBu-resistant MDA-MB-231 cell showed cancer stem cells character.

Figure 3

RT-PCR result indicate that the NaBu- resistant cell population displayes a higher expression level of MET (A). This was consistent with the western blot results (B). After seeding 105 cells in 100 mm peridish, NaBu- resistant cells showed the strongest sphere formation capability (2.7×104/dish) when compared with control siRNA treated goup(1.8×104/dish)and MET siRNA treated group(0.8×104/dish)(C). A higher CD133 expression level by western blot in the NaBu- resistant group was observed(D). More than 40% NaBu- resistant cells were positive for CD133 expression, however, within the control siRNA treated cells, only around 10% cell were positive for CD133(E). the CD133+ cells expressed higher level of c-MET compare to CD133 negtive cells(F). After treated with NaBu, cell viability of CD133+ cells are significantly higher than that of CD133- cells number . Intact MDA-MB-231 cells are used as control without the treatment of NaBu (G). *P<0.05.