Table 2. Growth parameters for ancestor, lacI and lacO1 mutants.
Lac→Glu | Glu→Lac | G+L→G+L | ||||||
Strain | Lag (hrs) | μMax (hrs−1) | Lag (hrs) | μMax (hrs−1) | Lag-1 (hrs) | μMax (hrs−1) | Lag-2 (hrs) | μMax (hrs−1)# |
Ancestor | 3.85±0.10 | 0.474±0.006 | 4.84±0.09 | 0.470±0.011 | 3.18±0.04 | 0.370±0.013 | 0.47±0.04 | 0.461±0.006 |
lacI | 3.83±0.09 | 0.468±0.007 | 3.59±0.07** | 0.484±0.009 | 3.12±0.09 | N/A | none | 0.495±0.009* |
lacO1 | 3.79±0.09 | 0.459±0.006 | 3.78±0.07** | 0.478±0.008 | 3.10±0.10 | N/A | none | 0.496±0.008* |
*Significantly different than ancestor, P<0.01 (two-tailed t-test, df = 10).
**Significantly different than ancestor, P<0.001 (two-tailed t-test, df = 10).
μMax for lacI and lacO1 is defined in an OD600 range that is most comparable with the OD600 range used to define μMax for the second exponential growth phase of the ancestor (μMax-2).