Figure 5. Acute loss of core integrin attachment complex members leads to collapse of attachments and multiple pathologies.

Collapse of attachment complexes into ball like structures was assessed (Figure S3) and compared with the severity of other pathologies associated with attachment complex disruption (Figure 1, Figure 2, Figure 3, Figure 4, and Figures S1 and S2). Pathologies identified as significantly different from matched controls (t = 72 h, P<0.001 two way repeated measures ANOVA) were placed into identical groupings based upon severity of defect. In the case of the movement defect the extent of severity was established as follows. First, the core complex members were considered as a group and examined against the remaining components for lack of significant difference from any member of the group (t = 72 h, P>0.05 one way ANOVA). Next, the remaining components were examined for groups of components where a significant difference between individual components within a group of components did not exist (t = 72 h, P>0.05 one way ANOVA) but a significant difference between every member of the group and all other components did exist (P<0.01, one way ANOVA). Thus, the colour coding for the extent of pathology as displayed in the inset legend reflects statistically significant differences in severity of defects.