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. 2012 Jan 12;8(1):e1002449. doi: 10.1371/journal.pgen.1002449

Figure 3. Gpr27 is required for mouse insulin promoter activity and glucose stimulated insulin secretion.

Figure 3

A. MIN6 cells were infected with either Ad-control or Ad-shGpr27. Three days after infection, RT-qPCR was performed for the indicated genes. The data are plotted as % expression compared to control adenovirus with standard error (n = 3). B. As in A, but dispersed primary mouse islets were infected with Ad-control or Ad-shGpr27. After 3 days, infected cells were sorted by flow cytometry of GFP positive cells and RT-qPCR was performed for the indicated genes (n = 3). C. MIN6 cells were infected with either Ad-control or Ad-shGpr27. Three days after infection, glucose stimulated insulin secretion was measured by ELISA after 1 hour of static incubation at either 2 mM or 20 mM glucose. Data are represented as the average of fractional insulin secretion with standard error (n = 9). *p<0.05, **p<0.005 versus Ad-control.