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pH dependence of binding. a. pH dependence of Kd for c-di-GMP (triangles) and pGpG (circles). Error bars for the pGpG values represent the standard deviation from at least three trials. The values for c-di-GMP are from a single measurement. b. The association constants (Ka=1/Kd) for pGpG as a function of pH. Error bars for the pGpG values represent the standard deviation from at least three trials.
