. Author manuscript; available in PMC: 2012 Jan 12.

Published in final edited form as: Biochemistry. 2011 Dec 27;51(1):425–432. doi: 10.1021/bi2016662

Table 1.

Binding affinities of linear analogs of c-di-GMP.

RNA	K_d (nM) c-di-GMP	K_d (nM) pGpG	K_d (nM) GpGp	K_d (nM) GpG	K_d (nM) pGpGp
Wild-type^a	0.01	490 ± 150	22,000 ± 14,000	210 ± 24	-
C92U	15 ± 1.1	-	-	> 100 μM^b	n.d.

All values are the average of three independent trials ± the standard deviation. A dash indicates that no binding was observed at 100 μM RNA. n.d. means not determined.

The wild-type RNA used here is the 110 Vc2 RNA. The C92U mutation was made in this RNA background.

A partial shift was observed at 100 μM RNA.