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Fluorescence anisotropy of unmethylated and methylated nucleosomes and the angle between the dipoles of the FRET pair.
| Excitation/Emission (nm) | 532/570 (Cy3) | 600/670 (Cy5) | 532/670 (Cy5 via FRET) | β (°) |
|---|---|---|---|---|
| UM601+39 | 0.33±0.01 | 0.33±0.01 | 0.07±0.01 | 46.7±0.97 |
| M601+39 | 0.34±0.01 | 0.33±0.01 | 0.13±0.01 | 39.7±1.12 |
