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PGC-1α was assessed by qPCR and western blot for potential transcriptional regulation of mitochondrial protein synthesis in liver (A), heart (B) and skeletal muscle (C). CR increased skeletal muscle PGC-1α at old age. There were no other differences in any tissue or at any age. *Significant difference between AL and CR, p ≤ 0.05, #Significant effect of age, p ≤ 0.05. n = 4–5 per group.
