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AMPK was assessed by qPCR and western blot for potential transcriptional regulation of mitochondrial protein synthesis in liver (A), heart (B) and skeletal muscle (C). P:T of AMPK was significantly greater in CR versus AL in liver and heart and in liver there was also a significant increase with age. In skeletal muscle there were no changes noted for AMPK. *Significant difference between AL and CR, p ≤ 0.05. n = 4–5 per group.
