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Assessment of global translation initiation by changes in RpS6 activation in liver (A), heart (B), and skeletal muscle (C). In general, there is a decrease in global translation initiation in all three tissues. These changes were reflected in total, phosphorylation or the ratio. *Significant difference between AL and CR, p ≤ 0.05, #Significant effect of age, p ≤ 0.05. n = 5 per group.
