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. 2011 Nov 28;11(1):104–110. doi: 10.1111/j.1474-9726.2011.00759.x

Figure 4.

Figure 4

Monocytes from older donors do not fully activate NF-κB pathway (A) A significant increase in IκBα degradation in monocytes from older donors. Densitometry of immunoblot of IκBα in monocytes from younger and older subjects before stimulation (n = 12/group) and after stimulation with Flagellin for 10 min (n = 21/group). Densitometry shows the means ± SEM of the ratio of total IκBα to β-actin. Asterisks indicate statistical significance between younger and older cohort (***P < 0.001). (B) Degradation of IκBα correlated with p38 phosphorylation in monocytes after stimulation with flagellin (n = 42, Pearson r = −0.5853, P < 0.0001). (C) The decrease in total NF-κB p65 and phospho-p65 expression in monocytes from older individuals. Densitometry of immunoblot of total p65 and phospho-p65 in monocytes from 21 pairs of younger and older subjects after stimulation with flagellin for 10 min. Densitometry shows the means ± SEM of the ratio of total p65 and phospho-p65 to β-actin. Asterisks indicate statistical significance between younger and older cohort (***P < 0.001).