TABLE 2. Conversion Factors between Allergen Standards Derived from Dose Response Curves or Analyses of House Dust Extracts.
Conversion Factors of Dose Response Curves (n=2)* |
Conversion Factors of Dust Extract Results** |
Mean Conversion Factors for ELISA Stds and UAS (#31012) |
||||
---|---|---|---|---|---|---|
Allergen | ELISA Std Lot # | UAS vs ELISA (i) | UAS vs ELISA (ii) | UAS vs ELISA | UAS to ELISA Std | ELISA to UAS Std |
Der p 1 | 2901 | 1.8 | 1.9 | 1.5 | 1.7 | 0.59 |
Der f 1 | 2762 or 30065 | 11.6 | 13.6 | 13.0 | 12.7 | 0.08 |
Der p 2 | 2409 | 1.0 | 1.0 | 2.3 | 1.4 | 0.71 |
Fel d 1 | 2853 or 30002 | 4.0 | 3.6 | 2.6 | 3.4 | 0.29 |
Can f 1 | 2832 | 5.4 | 6.3 | 5.9 | 5.9 | 0.17 |
Mus m 1 | 2508 | 1.0 | 1.0 | 1.2 | 1.1 | 0.91 |
Rat n 1 | 2714 | 0.4 | 0.5 | 0.5 | 0.5 | 2.10 |
Bla g 2 | 2418 | 1.8 | 1.8 | 1.4 | 1.7 | 0.59 |
ELISA dose response curves using the Universal Allergen Standard (Lot # 31012) or individual ELISA standards were compared in two separate assays (i) and (ii).
Dust extracts (n=13 to 18) were analyzed by ELISA using either UAS or individual ELISA standard. Values represent the mean correction factor of all the dust samples tested.