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. Author manuscript; available in PMC: 2013 Jan 11.
Published in final edited form as: J Am Chem Soc. 2011 Dec 9;134(1):305–314. doi: 10.1021/ja2071835

Figure 3.

Figure 3

The S. aureus AirS Fe-S cluster responds to O2, H2O2, and NO. (A) Anaerobically reconstituted AirS was purified through a desalting column and exposed to O2. UV-visible spectra were recorded before and after air exposure at various time intervals as indicated. (B) Effect of H2O2 on AirS. Reconstituted AirS (40 μM) was treated with H2O2 (80 μM and 2 mM) followed by UV-visible analysis. (C) UV-visible spectra of AirS (40 μM) before and after NO treatment (S-nitrosoglutathione (40 μM and 160 μM) was used as the NO donor). (D) Q-band EPR spectrum (g = 2.036) indicated the formation of a dinitrosyl-iron-dithiol (DNIC) complex.