Skip to main content
. Author manuscript; available in PMC: 2013 Jan 1.
Published in final edited form as: Aging Cell. 2011 Nov 28;11(1):93–103. doi: 10.1111/j.1474-9726.2011.00762.x

Fig. 5. The knockdown of rpi rescues the polyglutamine-induced rough eyes, and the overexpression of rpi does not further deteriorate the rough eye phenotype.

Fig. 5

The eye phenotypes resulting from different combinations of different transgenes of 127Q, 108Q, rpi, G6PD, GFP constructs are shown from A to O. (A) One copy of GMR-Gal4 displayed normal eye appearance as a control. (B) The expression of UAS-127Q by GMR-Gal4 exhibits rough eye phenotype. (C) The expression of UAS-hdj1 rescues the rough eye phenotype (Kazemi-Esfarjani & Benzer 2000), which was used as a positive control. (D, E) The RNAi knockdown of rpi by the transgene on the second (II) or the third (III) chromosome rescues the 127Q-induced rough eye. (F) The expression of UAS-108Q shows similar rough eye phenotype. (G, H) The RNAi knockdown of rpi also rescues the 108Q-induced rough eye. (I) The knockdown of rpi with two copies of UAS-rpiRNAi rescues the rough eye much better than a single copy of UAS-rpiRNAi. (J) The 108Q-induced rough eye cannot be rescued by two copies of UAS-GFP. (K) Knockdown of G6PD blocks the rescue by rpi knockdown on 108Q-induced rough eye. (L) Knockdown of G6PD does not degenerate the rough eye. (M) Overexpression of two copies of UAS-G6PD partially rescues the rough eye. (N) Overexpression of one copy of UAS-rpi does not further worsen the 127Q-induced rough eye. (O) The expression of two copies of UAS-rpi still does not deteriorate the rough eye.