Figure 2.

Decreased Aβ accumulation in human APOE haploinsufficient mice. Cortical tissues from 3-month-old APOE homozygous and hemizygous mice were sequentially homogenized with PBS and RIPA. Aggregated forms of Aβ in the RIPA-insoluble pellet were solubilized with 5 m guanidine HCl buffer. A, B, RIPA-insoluble Aβ40 (A) and Aβ42 levels (B) were measured from APOE3/3 and APOE3/− mice. C, D, Similarly, RIPA-insoluble Aβ40 (C) and Aβ42 levels (D) were measured from APOE4/4 and APOE4/− mice. (n = 12–20 per genotype).