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. Author manuscript; available in PMC: 2013 Jan 1.
Published in final edited form as: Bioorg Med Chem. 2011 Nov 23;20(1):47–57. doi: 10.1016/j.bmc.2011.11.032

Table 1.

Crystallization conditions

HPPK•17 HPPK•18 HPPK•19
PDB Entry Code 3UD5 3UDE 3UDV
Protein Solution
 HPPK (mg/mL) 10 10 10
 Compound 17 Saturated
 Compound 18 Saturated
 Compound 19 Saturated
 Tris-HCl [mM (pH)] 20 (8.0) 20 (8.0) 20 (8.0)
Reservoir Solution
 PEG 3350 [%(w/v)] 25 25 20
 CH3·COONH4 (mM) 200 200
 Bis-Tris [mM (pH)] 100 (6.5) 100 (8.5)
 HEPES [mM (pH)] 100 (7.5)
Crystals
 Appear (days) 7 7 14
 Reach final size (days) 14 14 21
 Shape Thin plate Thin plate Thin plate
 Dimension (mm) 0.15 × 0.10 × 0.005 0.10 × 0.05 × 0.005 0.15 × 0.10 × 0.005