Table III.

Ratio measurement values of kinetochore protein copy numbers in the DT40 cell line during metaphase based on our measurements for ScNdc80-GFP

Protein name	Average number per kinetochore	Average number per kMT
CENP-C	108 ± 35	25.0 ± 8.2
CENP-T	77 ± 20	17.9 ± 4.6
CENP-I	68 ± 17	15.9 ± 3.9
CENP-H CMV	79 ± 20	18.5 ± 4.7
CENP-H (KI)	73 ± 19	17.0 ± 4.5
Mis12	106 ± 32	24.6 ± 7.6
Knl/Blinkin	86 ± 23	19.9 ± 5.4
Nuf2	102 ± 30	23.7 ± 6.9
Hec1 (Ndc80)	90 ± 24	21.0 ± 5.5

Average protein copy numbers per kinetochore for DT40 cells were obtained by multiplying the average number of ScNdc80-GFP in an anaphase cluster, 306 ± 62, by the fluorescence ratio of ScNdc80-GFP to each GFP fusion protein measured previously for DT40 cells in Johnston et al. (2010). The average copy number per kinetochore was divided by the average number of microtubule attachments per kinetochore to obtain the average protein copy numbers per kMT in DT40 cells (4.3; Ribeiro et al., 2009).