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. 2011 Nov 14;195(4):605–615. doi: 10.1083/jcb.201105006

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© 2011 Morin-Leisk et al.

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Figure 1. — Identification of ATL2 middle domain residues required for its ER network branching function. (A) Knockdown replacement assay. 48 h after transfection with a Myc-tagged DP1 negative control (neg con) construct, wild-type HA-ATL2, or each of the indicated HA-tagged ATL2 variants, cells were transfected with siRNAs targeting ATL2 and ATL3. 72 h after knockdown, cells were fixed and stained using an antibody against the Myc or HA epitope and viewed by confocal microscopy. Bar, 10 µm. (B) Quantification of the fraction of cells expressing the indicated proteins that had the unbranched ER phenotype. Values represent the means of three independent experiments ± SD. *, P < 0.0005 with respect to wild type; **, P < 0.0005 with respect to the Myc-DP1 negative control.