Table II.
RSP molecular masses and estimated volumes in C. reinhardtii
RS domain | RSP | MM I 2D-NEPHGEa | MM II theoreticala | Estimated number of RSP copies | Integrated MM I/integrated MM II | Estimated volume for integrated MM I/MM II | Volume from reconstruction |
kD | kD | kD | nm3 | nm3 | |||
Head | RSP1 | 123 | 78.6 | 4 | 1,264/920.8 | 1,500/1,100 | 1,470 |
RSP4 | 76 | 49.8 | 4 | ||||
RSP6 | 67 | 48.8 | 4 | ||||
RSP9 | 26 | 29.5 | 4 | ||||
RSP10 | 24 | 23.5 | 4 | ||||
Neck | RSP2 | 118 | 77.4 | 2 | 508/354.8 | 600/420 | 600 |
RSP16 | 34 | 39 | 2 | ||||
RSP23 | 102 | 61 | 2 | ||||
Stalk | RSP3 | 86 | 56.8 | 4 | 1,477/1,294.5 | 1,750/1,530 | 1,600 |
RSP5 | 69 | 55.9 | 2 | ||||
RSP7 | 58 | 55 | 2 | ||||
RSP8 | 40 | 40.5 | 1 | ||||
RSP11 | 22 | 21.5 | 2 | ||||
RSP12 | 20 | 19.7 | 2 | ||||
RSP13 | 98 | 98 | 1 | ||||
RSP14 | 41 | 28.3 | 1 | ||||
RSP15 | 38 | 38 | 1 | ||||
RSP17 | 124 | 98.5 | 1 | ||||
RSP18 | 210 | 210 | 1 | ||||
RSP19 | 140 | 140 | 1 | ||||
RSP20 | 18 | 18.3 | 4 | ||||
RSP21 | 16 | 16 | 1 | ||||
RSP22 | 8 | 10.3 | 2 |
MM, molecular mass; NEPHGE, 2D nonequilibrium pH-gradient gel electrophoresis.