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. 2011 Oct 26;3(1):3. doi: 10.1186/1868-7083-3-3

Figure 3.

Figure 3

Sulforaphane decreased promoter methylation of cyclin D2. Methylation-specific PCR was performed after bisulfite modification of DNA from LnCap cells treated for 48 h. Upper figures are representative bands. UM indicates unmethylated cyclin D2 PCR products. M indicates methylated PCR products. The lower figure shows the levels of unmethylated versus methylated cyclin D2 PCR products by densitometry based on biological triplicates. SFN and 5-Aza-dC both decreased cyclin D2 promoter methylation. P values represent treatment values compared to vehicle.