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. 2011 Nov 28;195(5):889–902. doi: 10.1083/jcb.201104040

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© 2011 Karotki et al.

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Figure 4. — Structure of membrane-bound Pil1 and Lsp1. (A) Structure of Lsp1 and Pil1 bound to PI(4,5)P₂-containing liposomes. Tomographic midsections show that both proteins decorate liposomes and constrict them to a similar diameter. Bar, 50 nm. (B) Classification of Lsp1 filament segments in addition to membrane-bound Lsp1 and Pil1 reveals different diameters. N represents the number of segments used for the classification. (C) Helical reconstruction of prominent groups of Lsp1 filaments as well as membrane-bound Lsp1 and Pil1. Membrane-bound Lsp1 shows distinct density oriented toward the lipid bilayer. Bar, 10 nm.