Figure 7.

Eisosomes in situ structurally resemble Pil1 and Lsp1 assemblies. (A) Representative image of the yeast plasma membrane from the cytosolic side (top). Bar, 300 nm. (insets) Magnifications of distinct areas (marked by white boxes) of the membrane show striated areas (red parallel lines) that resemble the pattern of recombinant Pil1 and Lsp1 structures. Bars, 100 nm. (B) Immunolabeling of plasma membranes of cells expressing Pil1-GFP using anti-GFP antibodies. Yellow circles highlight 18-nm gold particles for better visibility. Bars, 100 nm. (A and B) The structures are visible on the flat membrane as well as on the side of large invaginations (arrowheads). (C) DEEM images showing views on the plasma membrane from different perspectives. (top) View from the outside of a cell onto the inner leaflet of the plasma membrane. (bottom) View from the cytoplasm (marked as c) onto the plasma membrane (marked as m; red/cyan 3D glasses are recommended for 3D view, as well as for D). Bars, 300 nm. (D) View from the cytoplasm onto an eisosome at the plasma membrane. Arrowheads indicate how the plasma membrane protrudes underneath the eisosome protein coat to form a groove instead of a closed tube. Bar, 100 nm.